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This system involved the administration of anabolic steroids on rats, either orally or by injection (depending on the anabolic steroid being assessed)and the administration of a single dose of the non-steroidic levothyroxine (L-thyroxine) at a single time point (a fixed time line for each treatment), at the same time of the day (morning, afternoon, or evening). Although the time of day used was not controlled for, and all conditions were identical – rats were allowed to eat, drink and bathe, regardless of whether their food was available or not – at the end of each treatment period, the animals were allowed to remain in the study (at home and in the testing room) for two hours. The total treatment period of each study period was 1 week and was divided into days of the week, with 1, 2, 3, the weekend, and an additional 2, 4, 5, 6, 7, and 8 day break periods (days of the week). The study period was divided into four treatment sessions: 1 day on which there was baseline testing, 2 days where testing took place on the first two days of the study (with or without L-thyroxine); 3 days on which L-thyroxine was given; 4 days where testing took place on the third, fourth, and fifth days of the study – where the rats were allowed to remain in water for 15 min after ingestion; and 5 days when blood was taken. Testing took place on each animal, and a minimum of 1 test day was always included for each treatment session. The order in which tests were carried out was as follows: blood collection for evaluation of testosterone (F) in the plasma (T) at 20 minutes after oral L-thyroxine was given (T+OD) [ 20 ]; LH (LH) in the plasma (LH) at 20 minutes after oral l-thyroxine was given (LH+OD, T+LH); and testosterone (T) and LH (LH)+LH (T+LH) at 1, 5, 10 and 15 minutes after oral l-thyroxine (T+5OH+OD, T+5OH−OD, T+10OH+OD); and l-thyroxine (L-Th) in the urine (L-TL) at 10 minutes after oral l-thyroxine (L-TL +OD, L-TL +5OH) was given (L-TL +LH+OD, L-TL +LH+5OH). Testosterone (F) Similar articles: